WASHING WITH ALKALINE SOLUTIONS IN PROTEIN A PURIFICATION IMPROVES PHYSICOCHEMICAL PROPERTIES OF MONOCLONAL ANTIBODIES

Washing with alkaline solutions in protein A purification improves physicochemical properties of monoclonal antibodies

Abstract Protein A affinity chromatography has been widely used for both laboratory scale purification and commercial manufacturing of monoclonal antibodies and Fc-fusion proteins.Protein A purification is specific and efficient.However, there still remain several issues to be addressed, such as incomplete clearance of impurities including host cel

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Structural design and sealing performance of a composite wear-resistant cup for pipelines with diameter of 355.6 mm

In order to reduce the partial abrasion of the cup of pig and detector in pigging and detection under some special conditions, the causes for partial abrasion of cup were analyzed in depth, a structural design scheme of PRO OREGANO+BLACK CUMIN OIL composite wear-resistant cup for oil and gas pipeline with diameter of 355.6 mm was proposed, and the

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